In operations conducted Tuesday and Wednesday, the Traffic and Road Safety department found motorists without either headlights or interior lights, which is against traffic rules.
Among those impounded and owners penalised include 160 motorcycles and 98 vehicles.
Headlights for motorcycles moving on public road are required to be on at all times while interior lighting in public service vehicles is mandatory when in operations at night for the security of passengers.
Other automobiles had no headlights at night.
This comes after other 200 vehicles and motorcycles were impounded on Monday, when lighting enforcement was commenced following mass awareness on lighting regulations.
"All measures are being taken to ensure road safety and security of the people, including lighting.
Those violation these regulations will be penalised accordingly," Commissioner of Police CP John Bosco Kabera the RNP spokesperson, said.
He added: "It is easy to identify a motorcycle during the day and night, when its headlights are on. Interior lighting in public service vehicles is also mandatory to ensure the security of passengers but it’s also a general rule that all automobiles must turn-on headlights at night."